A method for determining Mg2 ++ in red blood cells of peripheral blood, which allows to determine oxygenation of hemoglobin in the background of acute cytomegalovirus infection

摘要

A method for determining the peripheral blood erythrocytes Mgv comprising performing histochemical reaction in the incubation solution consisting of 500 .mu.l of distilled water with the addition of 250 l of 10% sodium tungsten and 0,67N 250 l sulfuric acid in an amount which is placed in a 625 ul tube was added thereto and and a drop of methyl red 0,2N NaOH until a yellow color, and then introduced into the tube 250 l of 2% gidroksilaminogidrosulfata, 250 .mu.l of 0.075% titanium yellow and 500 l 1,5N NaOH, and then introduced into a separate tube 250 .mu.l of the resulting solution and 250 l of whole blood coagulant, incubated for 15 min at room temperature, is prepared monolayer smear centrifuge «Diffe Spin-2", dried at room temperature and examined under a digital microscope combined with «SCION Corporation» (USA) computer cytophotometry program allowing in a single red blood cell to determine the density of the reaction in conventional units and by increasing the number of red blood cells with a response to Mgdo 60,0 ± 2,9% to the activity of the reaction in a single red blood cell to 75,30 ± 1,85 conv. u point decrease in titre when to cytomegalovirus 1: 1600 reduction in oxyhemoglobin in erythrocytes to 91,5 ± 1,3%, it becomes a threat to the formation of a pregnant anemia, so the proposed method makes it possible to solve the diagnostic and research tasks.