METHOD OF INDUCING CYTOTOXIC ANTI-TUMOUR IMMUNE RESPONSE in vitro WITH USING DENDRITIC CELLS, TRANSFECTED BY RNA TUMOUR CELLS, TO NON-SMALL CELL LUNG CANCER

摘要

FIELD: biotechnologies.;SUBSTANCE: invention can be used for inducing an antitumour immune response in vitro. Method involves production of adhesive and non-adhesive fractions of mononuclear cells (MNC) recovered from patient's peripheral blood, combined cultivation recovered from patient's peripheral blood MNC non-adhesive fraction with mature dendritic cells (DC), made from adhesive MNC fraction, transfected with RNA of tumour cells. Then, DC is subjected to stimulated ageing. Combined cultivation non-adhesive MNC fraction and transfected RNA autologous tumour cell DC is performed for 5 days. Note here that for producing mature DC after three days of cultivation of adhesive MNC fraction are transfected immature DC tumour RNA, and one day after transfection immature DC is for days stimulated ageing by adding a culture of immature DC tumour necrosis factor-α (TNF-α) and Interleukin-1β (IL-1β) in the following proportions: TNF-α : IL-1β= 2.5:1.;EFFECT: invention allows to effectively induce cytotoxic anticancer immune response to non-small cell lung cancer in vitro with the help of dendritic cells, transfected by RNA tumour cells.;1 cl, 1 tbl