Method for identifying ligands for sigma-1 receptors

摘要

A fusion protein comprising (i) a receiver σ1 or a functionally equivalent variant thereof, wherein said functional equivalent σ1 receptor has an identity amino acid sequence of at least 70% with the human receptor σ1 wildtype and wherein said functional equivalent of σ1 receptor has the ability to bind to haloperidol with an affinity of at least 10% of the human σ1 wild type receptor, (ii) a residue of donor fluorescent protein, and (iii) a residue of fluorescent protein acceptor, wherein said residues fluorescent protein donor and acceptor are capable of producing energy transfer fluorescence resonance (FRET) and wherein the σ1 receptor is flanked by other donor fluorescent protein and other fluorescent acceptor protein.