Procedure to detect the presence of broad-spectrum β-lactamase producing bacteria in a sample

摘要

A method for detecting the presence of broad-spectrum β-lactamase producing bacteria in a biological sample, said method comprising the steps of: a) carrying out cell lysis in a biological sample in order to obtain an enzymatic suspension; b) reacting a fraction of the enzyme suspension obtained in step a) with a reagent kit, said reagent kit comprising - a broad-spectrum β-lactamase substrate that is selected from the group consisting of cephalosporins, aztreonam and cephamycins, and - a color pH indicator that will change color when the pH of the reaction mixture is between 6.4 and 8.4, in which a color change after step b) indicates the presence of broad-spectrum β-lactamase producing bacteria in the biological sample in which said biological sample is a blood sample or a urine sample