Acidified seawater responsive genes in Aurelia aurita and the prediction method for physiological or metabological changing of Scyphozoa

摘要

The present invention relates to an Aurelia aurita gene responding to acidified seawater exposure, and to a method for diagnosing a change in an aquatic ecosystem environment by using the same. In particular, a polyp of Aurelia aurita is cultured, and is exposed to acidified seawater of pH 7.5 for 6, 24, and 48 hours. RNA is extracted therefrom, and cDNA is synthesized. Then, hybridization is performed by marking the same by Cy3 and Cy5, and a microarray analysis is performed by utilizing an Aurelia aurita gene Hazchem fish array. As a result, it has been confirmed that the expression of 33 genes in an acidified seawater (pH 7.5) 6-hour exposure group, the expression of 36 genes in a 24-hour exposure group, and the expression of 96 genes in a 48-hour exposure group are changed by two or more times. Also, it has been confirmed that the expression amounts of total 165 genes excluding overlapping genes are changed by exposure to acidified seawater. Accordingly, the genes can be usefully used as a biomarker for confirming the exposure of acidified seawater, and can be usefully utilized in predicting the hypertrophied of jellyfish according to a change in the marine environment.