ADAPTED PANDEMIC INFLUENZA VIRUS STRAINS A/Tomsk/273/2010-MA1(H1N1pdm09), A/Tomsk/273/2010-MA2(H1N1pdm09) AND A/Tomsk/273/2010-MA3(H1N1pdm09) TO ASSESS THE EFFECT OF ANTIVIRAL DRUGS (VERSIONS)

摘要

FIELD: medicine.;SUBSTANCE: invention relates to general and medical virology and concerns influenza A virus. Obtaining new adapted versions of pandemic influenza A(H1N1)pdm09 strain to organisms of different laboratory animals, proposed three adapted versions of the pandemic influenza virus obtained from wild-type strain A/Tomsk/273/2010(H1N1pdm09) by multiple passing through lungs of experimental mice. Strain A/Tomsk/273/2010-MA1(H1N1pdm09) passed 7 passages through lungs of mice of line BALB/c, strain A/Tomsk/273/2010-MA2(H1N1pdm09) - 12 passages through lungs of mice of line C57BL/6z, and strain A/Tomsk/273/2010-MA3(H1N1pdm09) - 10 passages through lungs of mice of line CD1. All the above strains cause 100 % death rate among mice, to organisms of which they are adapted. Strain A/Tomsk/273/2010-MA1(H1N1pdm09) is high-fatal not only for mice, to the body of which it was adapted (mice of line of BALB/c), but for mice of opposite line C57BL/6z causing among the last 100 % lethality.;EFFECT: obtained strains can be used for studying of mechanisms of adaptation of viral pathogens to new host with evaluation of action of antiviral drugs in treating of influenza disease caused by pandemic VG A(H1N1)pdm09.;3 cl, 2 dwg, 5 tbl, 5 ex