RECOMBINANT PLASMID pET-15b_T1_RL DNA PROVIDING SYNTHESIS OF RECOMBINANT FUSION PROTEIN CONSISTING OF TUMOR-SPECIFIC PEPTIDES AND ANTITUMOR PEPTIDE RL2, AND RECOMBINANT FUSION PROTEIN POSSESSING CYTOTOXIC ACTIVITY AGAINST CANCER CELLS AND TARGETED PROPERTIES AGAINST TUMOR TISSUE

摘要

FIELD: biotechnology.;SUBSTANCE: pET-15b_T1_RL plasmid DNA is constructed which provides synthesis of recombinant fusion protein consisting of a tumor-specific peptide and an antitumor peptide RL2. The recombinant fusion protein isolated from Escherichia coli cells transformed with pET-15b_T1_RL plasmid, has a molecular weight of 15.7 kDa, consists of methionine residue, tumor specific peptide GLHTSATNLYLH, glycine GGGS spacer, and a recombinant lactaptin RL2 analogue.;EFFECT: production of a protein providing cytotoxic activity against cancer cells in culture and targeted properties for tumor tissue.;2 cl, 6 dwg, 5 ex