METHOD FOR PREPARATION AND APPLICATION OF AUTOLOGICAL ERYTHROCYTES FROM UMBILICAL BLOOD FOR ANEMIA CORRECTION IN NEWBORNS

摘要

FIELD: medicine.;SUBSTANCE: method includes the following steps: umbilical cord samples collection, processing, placement for storage and rejection of samples, a pre-transfusion processing for umbilical blood samples. At the pretransfusion preparation stage, the erythrocyte mass from the plasma is separated by centrifugation at acceleration of 3768 g for 15 minutes at a temperature of +4°C. Then the auto-erythromass samples pass the procedure of auto-erythrocytes washing from the preservative and hemolyzed cells formed during umbilical cord sample storage. To do this, 100 ml of sterile physiological sodium chloride solution is added to the packet with auto-erythromass in the umbilical erythromass and physiological solution ratio of 1:1 and centrifuged at 1971 g acceleration for 10 minutes at a temperature of +4°. After this, the supernatant is removed using a plasma extractor. The washed umbilical erythrocytes are filtered through a microaggregate filter. All samples of autologous erythrocytes of umbilical blood pass quality control: the degree of hemolysis and quantitative indicators of blood cells, hemoglobin and hematocrit are determined.;EFFECT: invention allows to correct anemia in newborns by a single blood transfusion, without occurrence of posttransfusion complications associated with donor blood components transfusion.;11 tbl, 3 ex