GENETIC CONSTRUCT BASED ON CRISPR/Cas9 GENOME SYSTEM EDITING, CODING Cas9 NUCLEASE, SPECIFICALLY IMPORTED IN HUMAN CELLS MITOCHONDRIA

摘要

FIELD: biotechnology.;SUBSTANCE: genetic construct of pMitoCas9 with SEQ ID NO:1 based on the CRISPR/Cas9 genome editing system is obtained, which encodes the cas9 nuclease, specifically imported into human mitochondria.;EFFECT: invention provides efficient synthesis of modified Cas9 nuclease in human cells.;2 dwg