method, method for producing an antibody, expression vector, method for selecting a recombinant mammalian cell, use of an expression vector, expression plasmid, one aspect, method for selecting an eukaryotic cell expressing an antibody, expression plasmid expression, use of an expression plasmid and expression plasmid or use or method

摘要

Herein is reported that for transient transfections the use of the human elongation factor 1 alpha promoter (with Intron A) provides for an enhanced productivity (in LC-HC-SM organization), the use of the bovine growth hormone polyA signal sequence provides for an enhanced productivity compared to use of the SV40 polyA signal sequence, the addition of the HGT to the bGH PolyA signal sequence results in an increased productivity in vectors containing the hCMV promoter and the vector organization LC(3'-5')-HC-SM results in improved expression. For stable pools it is reported that pools generated with vectors containing the hEF1± promoter show an enhanced productivity in batch analysis, clones generated with vectors containing the hEF1± promoter show a reduced number of low producing clones, and clones generated with vectors containing the hEF1± promoter show a higher stability of IgG expression. For single clones it is reported that the vector organization with downstream position of selection marker (LC-HC-SM) has a positive effect on productivity of single clones and that clones generated with vectors containing the bGH polyA signal sequence and the hGT have higher productivities.