THERMOSTABLE SUPEROXIDE DISMUTASE ENZYME PURIFIED FROM BLACK CARDAMUN (A. SUBULATUM) FOR CYTOTOXIC EFFECT ON HEP G-2; HUMAN LIVER CANCER CELLS

摘要

The present invention relates to thermostable superoxide dismutase (SOD) enzyme purified from black cardamum {A. Subulatum); a household spice; for cytotoxic effect on HEP G-2; human liver cancer cells. The enzyme isolated from Black cardamum after purification to apparent homogeneity and amino acid sequencing using MALDI-TQF-TOF analysis is found to .be novel. The apparently homogenous and thermostable SOD of the invention exhibits substantially high cytotoxicity against human liver cancer cells (Hep G-2).