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METHOD FOR RAPID DETECTION OF INFECTION FROM CAGA POSITIVE HELICOBACTER PYLORI AND DIAGNOSTIC KIT THEREFOR
METHOD FOR RAPID DETECTION OF INFECTION FROM CAGA POSITIVE HELICOBACTER PYLORI AND DIAGNOSTIC KIT THEREFOR
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机译:快速检测卡加阳性幽门螺杆菌感染的方法及其诊断试剂盒
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摘要
The invention relates to an in vitro method for the detection of the pres- ence of highly pathogenic CagA+ strains of Helicobacter pylori, or of H. pylori strains expressing the cag pathogenicity island, in samples of gastric mucus, feces or biopsies, which method provides results immediately available on performing the test, as well as the diagnostic kits for use in carrying out said method. The method exploits a coagglutination or latex agglutination test pro- cedure implemented by sensitizing with anti-CagA sera or with an antiserum anti-total protein of CagA-positive H. pylori from which the other antibodies against antigens of H. pylori have been separated, with a suspension of staphylococci with a high content of Protein A or with latex particles, and de- tecting the possible agglutination in biological samples examined.
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