Methods and apparatus for analyte collection, extraction, concentration, and detection for clinical applications

摘要

In various embodiments, devices and methods are provided for the detection and / or weighing of clinically relevant pathogens (eg, bacteria, fungi, viruses, etc.). In certain embodiments, the device has a bacterial concentration at a concentration of less than about 6 × 10 ⁶ cells / mL, less than about 3 × 10 ⁶ cells / ml, less than about 1 × 10 ⁶ CFU / mL, or less than about 50 μg / mL. A lateral flow assay to detect. In certain embodiments, the device comprises an aqueous two-phase system (ATPS) comprising a mixed phase solution that separates into a first phase solution and a second phase solution, and a lateral flow assay (LFA). ing. In certain embodiments, the device includes a flow-through system comprising a concentration component comprising an aqueous two-phase system (ATPS) comprising a mixed phase solution that separates into a first phase solution and a second phase solution. And a detection component disposed below the concentration component described above.