NOVEL PRIMER COMBINATION FOR AMPLIFYING, BY PCR, AND SEQUENCING MITOCHONDRIAL CO1 GENE USED FOR DNA BARCODING OF MARINE FISHES AND DNA SEQUENCING METHOD USING SAME

摘要

The present invention performs a polymerase chain reaction (PCR) amplification reaction of mitochondrial cytochrome c oxidase 1 (CO1) gene for DNA barcoding of marine fishes, and provides a primer combination where one or more of SEQ ID NOS: 1-3 for decoding a nucleic acid sequence is used as a forward primer and at least one of SEQ ID NOS: 1-3 is used as a reverse primer, thereby enhancing a success rate and efficiency of the PCR amplification reaction to facilitate identification of various species, and decoding longer nucleic acid sequences in PCR amplification products obtained therefrom, which can be used for molecular genetic studies of marine fishes.;COPYRIGHT KIPO 2019