Method of embryogenic tissue induction from immature zygotic embryo in Japanese larch

摘要

The present invention comprises the step of surface sterilization by separating the immature seeds contained in the immature conifer of the collected Japanese Larch, and after removing the seed of the surface sterilized immature seeds, by cutting the immature seeds from which the seed is removed in longitudinal section It provides a method of inducing embryogenic tissue from seed immature embryos of Japanese larch tree, comprising the steps of nutrients, and culturing the nutrient-cut cut surface to contact the embryogenic tissue induction medium.