OLIGONUCLEOTIDE DNA PRIMERS FOR REVEALING AND PHYLOGENETIC ANALYSIS OF THE CAT LEUKOSIS VIRUS, AS WELL AS FOR THE SYNTHESIS OF THE GENE FOR GLYCOPROTEIN GP70 CONTAINING PROTECTIVE ANTIGENIC EPITOPES

摘要

FIELD: biotechnology.;SUBSTANCE: invention relates to biotechnology. Proposed oligonucleotide DNA primers for the detection and phylogenetic analysis of the proviral DNA of feline leukemia virus, as well as for the synthesis of a fragment of the surface glycoprotein gp70 gene containing protective antigenic epitopes, characterized in that two pairs of oligonucleotide DNA primers have the following sequences: external (1 fwd: 5'TCCAACGCACCCAAAACCCT3'; 1 rev: 5'GCTTTAGTCCCTGTTCCGAC3'), internal (2 fwd: 5'TCCTGCCTATCTACTCCGCA3'; 2 rev: 5'ATGCATGGGGTGAGTCCAGT3'). Invention can be used diagnostic veterinary laboratories, research institutions, biotechnology companies.;EFFECT: synthesis of the target DNA fragment, the coding fragment of the surface glycoprotein gp70 feline leukemia virus, which can be used for phylogenetic analysis and synthesis of viral antigen.;1 cl, 1 dwg, 3 tbl, 3 ex