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Low-retention pre-columns: a straightforward approach to enable larger injection volumes and reduce extra-column effects in HPLC
Low-retention pre-columns: a straightforward approach to enable larger injection volumes and reduce extra-column effects in HPLC
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机译:低保留预柱:一种简单的方法,可实现更大的进样量并减少HPLC中的柱外影响
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摘要
Low-retention pre-columns that allow increased injection volumes of solvents chromatographically stronger than the mobile phase and use of solvents with limited solubility in the mobile phase, such as ethyl acetate and MTBE. The system and method also reduces band broadening due to the extra-column effects acting upstream of the analytical column, including band broadening due to the injection process and due to the connecting tubing and fittings between the injection system and the column. A pre-column may also be used as a guard column, thereby minimizing band broadening due to the guard column.
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