The present disclosure discloses a GPU-based TFT-LCD Mura defect detection method, comprising: (1) establishing a studentized residual based double-second-order regression diagnosis model based original image data to obtain double-second-order regression background data; (2) obtaining influence quantities of respective data points on fitted values according to the original image data and the double-second-order regression background image data; (3) excluding outliers and influential points in the original image data according to the influence quantities to obtain a new pixel point set; (4) establishing a double-N-order polynomial surface fitting model according to the new pixel point set to obtain double-N-order background image data; (5) obtaining a residual image R according to the double-N-order background image data and the original image data, and performing threshold segmentation on the residual image to obtain a threshold segmentation image; and (6) performing morphological processing on the threshold segmentation image to obtain an eroded and dilated image, thereby achieving effective segmentation of Mura defects with uneven brightness distribution.
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