PROCESS FOR THE PRODUCTION OF A PERMANENT HUMAN AMNIOCYTE CELL LINE, PROCESS FOR THE TRANSITIONAL EXPRESSION OF POLYPEEPTES AND RECOMBINANT PROTEINS WITH THE USE OF THE REFERRED CELL LINE, AND THE USE OF THE REFERRED CELL LINE.

摘要

process for the production of a permanent human amniotic cell line, human aminocytic cell line, process for the transient expression of recombinant polypeptides and proteins with the use of a line, use of cells and polypeptide or protein. the present invention relates to a permanent human cell line, comprising the nucleic acid sequences for the adenoviral genetic functions e1a and e1b and the nucleic acid sequence for the sv40 large t antigen or the epstein virus nuclear 1 antigen -barr (ebv) (ebna-1). the present invention also relates to a process for the transient expression of recombinant polypeptides and proteins in the permanent human cell line.