ULTRAVIOLET LIGHT TRIGGERED CROSSLINKING NEAR-INFRARED MOLECULAR PROBE AND PREPARATION METHOD AND APPLICATION THEREFOR

摘要

Provided are an ultraviolet light triggered crosslinking near-infrared molecular probe and a preparation method and an application therefor. The preparation method comprises the following steps: constructing and synthesising a photo-crosslinking near-infrared molecular probe DACF; triggered by ultraviolet light, the photo-crosslinking near-infrared molecular probe DACF implements crosslinking in cells. The probe is irradiated by light of a specific wavelength to produce a highly active carbene intermediate that bonds covalently to intracellular biomolecules, significantly increasing the intake of the probe in the cells and extending the retention time, and being capable of implementing long-term tracking of tumour cells. In addition, using the photothermal effect of the probe can better kill tumour cells. The photo-crosslinking near-infrared molecular probe DACF obtained by the present preparation method can effectively increase the enrichment and retention time of the probe in living tumours, integrating the diagnosis and treatment of tumours, and having important scientific research and economic value.