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THREE-DIMENSIONAL CULTURE METHOD, THREE-DIMENSIONAL CULTURE STRUCTURE, AND THREE-DIMENSIONAL CULTURE STRUCTURE MANUFACTURING METHOD

机译:三维文化方法,三维文化结构和三维文化结构制造方法

摘要

This three-dimensional culture method includes: a step for preparing a cell suspension containing a cell (12C) and a culture medium (14M); a step for preparing a solid surface (10S) that has a plurality of protrusions (10Sp) each having a height of 10nm-1mm; a step for adhering a drop (16D) of the cell suspension onto the solid surface (10S); and a step for culturing the cell (12C) in the drop (16D), in a state where the direction of gravity acting on the drop (16D) is towards the solid surface (10S).
机译:该三维培养方法包括:制备包含细胞(12C)和培养基(14M)的细胞悬液的步骤;和制备具有多个突起(10Sp)的固体表面(10S)的步骤,每个突起具有10nm-1mm的高度;将细胞悬液的液滴(16D)粘附到固体表面(10S)上的步骤;以及在作用于液滴(16D)上的重力方向朝向固体表面(10S)的状态下在液滴(16D)中培养细胞(12C)的步骤。

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