TEST SYSTEM FOR IDENTIFICATION OF DNA OF DOMESTIC DOG TISSUE (CANIS LUPUS FAMILIARIS) IN DRY FODDER AND SEMI-FINISHED MEAT PRODUCTS

摘要

FIELD: biotechnology.;SUBSTANCE: invention relates to the field of biotechnology. Invention presents a test system for identification of DNA of domestic dog tissue (Canis lupus familiaris) in dry fodders and semi-finished meat products, including a polymerase chain reaction buffer, a mixture thereof, consisting of deoxynucleoside triphosphates, oligonucleotide primers and fluorescent probes specific for the region of the animal genome and for the internal control sample; mixture of enzymes from DNA polymerase with antibodies inhibiting enzyme activity, TAQ POLYMERASE, internal control sample in form of suspension of bacteriophage T4 with concentration of 5 × 10³ phage particles per 1 mcl, a negative control sample representing a mixture of recombinant plasmid DNA containing a fragment of the animal's genome and a fragment of the bacteriophage T4 genome with a nucleotide sequence: T4F: 5' - TACATATAAATCACGCAAAGC-3' - forward primer; T4R: 5' - TAGTATGGCTAATCTTATTGG-3' - reverse primer; T4P: SU5-5' - ACATTGGCACTGACCGAGTTC-3'-BHQ1 - probe, taken in volume ratio of 1:1, according to invention for internal control sample is phagolysate of bacteriophage T4, and for a positive control sample fragments of genomes of native bacteriophage T4 and domestic dog (Canis lupus familiaris) with the following nucleotide sequence are used: Canis F ATTCggCCTACATCCgTgAC forward primer; Canis R AgAAgACCCCTgCTACgACT reverse primer; Canis P FAM-CTTgAgTggAgTAgggCgg - BHQ1 probe.;EFFECT: invention widens functional capabilities and accuracy of identification of species identity, simplifies the sampling process.;1 cl, 5 tbl