PROCÉDÉ DE PURIFICATION D'EXOTOXINE A RECOMBINANTE, MUTÉINES D'EXOTOXINE A RECOMBINANTE, ET PROTÉINES RECOMBINANTES COMPRENANT DES FRAGMENTS D'EXOTOXINE A

摘要

A method for purifying a protein comprising an Exotoxin A, Exotoxin A mutein or fragment thereof, the method comprising:(a) solubilizing a sample comprising the protein in a solubilization buffer containing a low concentration of urea and at an alkaline pH,and (b) decreasing the concentration of urea and the pH by dilution using a dilution buffer, and / or by dialysis into a dilution buffer,prior to purification of the protein.