Phosphorylation of the Butyrate-Enhanced Protein, Histone H1 exp 0 , in Chinese Hamster Cells

摘要

Although the model studies focus attention upon the ability of H1 phosphorylation to alter H1-DNA interactions, the molecular effects of H1 phosphorylation in chromatin remain unresolved. Part of the difficulty has been technical. For example, most H1 molecules become dephosphorylated during the isolation of chromatin and chromosomes; therefore, it has not been possible to compare the properties of fully phosphorylated chromatin or chromatin subunits with those of their unphosphorylated counterparts. Recent reports, however, suggest that it may be possible to replace unphosphorylated H1 with phosphorylated H1 in oligonucleosomes, nucleosomes, and chromatosomes. Hence, in the near future, we anticipate significant advances in this area. (ERA citation 05:038742)