Proteomics Characterization of the Molecular Mechanisms of Mutant P53 Reactivation with PRIMA-1 in Breast Cancer Cells

摘要

The main purpose of the study is to identify novel protein-protein interactions in various locations of cells to establish the molecular mechanisms of mutant p53 reactivation with PRIMA-1 in breast cancer cells. To achieve this goal, co-immunoprecipitation/mass spectrometry approaches are used to search for novel proteins that interact with p53 in the cytoplasmic and nuclear fractions of cells. The identity of interacting proteins are validated and confirmed by immunoblot analyses and protein translocation is detected by confocal microscopy. Our approach has identified hsp90 as a partner protein that is associated, in part, with the restoration of p53 transcriptional transactivation function of PRIMA-1.