Dissecting Androgen-Dependent and Independent Signaling Pathways Using RNA Interference-Based Functional Genomics in Human Cells

摘要

We had previously identified androgen responsive genes in LNCaP prostate cancer cells using microarray technology. I have performed a high throughput loss of function screen using RNA interference (RNAi) in order to identify androgen responsive genes that are critical for androgen induced proliferation. I am currently investigating whether the genes identified in my screen function in prostate tumor progression. At the same time, in collaboration with scientists from the Broad Institute of Harvard and MIT, we screened by RNAi LNCaP cells among a panel of human cancer cell lines to identify synthetic lethal partners of oncogenic KRAS, and identified TBK1 as a gene that is essential in cells with mutant KRAS. The results from this study are currently in review for publication.