Industrial Propagation of Chukrasia Tabularis A. Fuss by Bioreactor Technique

摘要

Background: Chukrasia Tabularis A. Fuss is a valuable woody plant and difficult in reproduction in nature. Objectives: There are a needs to conserve and develop for re-forest. Method: Manipulation of tissue culture via bioreactor techniques was the effective new way to resolve re-forest. Stems and leaves of in vitro plantlets were used as planting materials. Somatic embryo callus was initiated on medium MS + BA (2mg/l) + PVP (500 mg/l) supplemented with 2,4D (2mg/l). Somatic cell suspension was cultured for initiation and for proliferation. On medium MS + 2,4D (2mg/l) supplemented with BA (1mg/l). The volume of somatic cell suspension for bioreactor cultivation was 20. Somatic embryo suspension was cultured in bioreactor for initiation and proliferation on the medium MS + 2,4D (2mg/l) supplemented with BA (1mg/l). Embryogenic suspension was stimulated on the medium MS + 2,4D (1mg/l) + CW (10) + sucrose (45g/l) supplemented with BA (0.5mg/l) + NAA (0.1mg/l). In vitro shoots of Chukrasia Tabularis a. Fuss were regeneration on the medium MS + 2,4D (1mg/l) + CW (10) + sucrose (45g/l) supplemented with TDZ (0,5mg/l). Plantlets were enhanced growth and development in immersion-bioreactor cultivation by sinking/rising floated 1min/4hrs. Temperature, light intensity and stirring in stirring-bioreactor cultivation were favored at 262, 11,1-22,2mol/m~2/s, and 30rpm. Results: Micropropagation of Chukrasia Tabularis a. Fuss by bioreactor technique was set up to produce 4,680 plantlets per one liter of somatic embryogenesis suspension Conclusion: a process of rapid industrial multiplication of woody plants by bioreactor technology has been built.