Alternative method for direct DNA probe labeling and detection using the checkerboard hybridization format.

摘要

Molecular diagnostic methods using genetic material probes have been employed in the health care field for the detection and quantitation of several species of microorganisms (2, 5). These methods are faster and more suitable than traditional culture methods. In addition, the possibility of the inclusion of difficult-to-culture, uncultivable, or uncharacterized species in the set of target species has led to a more comprehensive investigation of microorganism communities in oral infections (1. 7).