Expression pattern of ZNF33B in bovine ovaries and the effect of its polymorphism on superovulation traits

摘要

ZNF33B belongs to recently duplicated Kruppel-associated box domain zinc finger proteins (KRAB-ZFPs), which is widely present in various organs, and some evidence showed that its expression is altered in the ovary undergoing superovulation. In this study, the expression of ZNF33B in ovary and early embryo was determined by immunohistochemistry and immunofluorescence techniques. Results showed that the expression of ZNF33B in the ovary was mainly in the cytoplasm of oocytes and granulosa luteal cells of ovarian corpus luteum and significantly reduced during follicular ovulation to luteal degeneration. The expression of ZNF33B in the early embryo transferred from the nucleus to the whole cell, suggesting that the expression of ZNF33B is spatiotemporally specific. Then, in combination with the single nucleotide polymorphism (SNP) database, the g.-61G>T mutant of the 5 '-untranslated region (5 ' UTR) of the ZNF33B gene was screened out from 556 Changbaishan black cattle, and the frequency of the mutant gene was counted. The statistics of superovulation and superovulation traits confirmed significant differences between the two genotypes in the quantity and quality of oocytes obtained after superovulation. This study confirmed, for the first time, the effect of ZNF33B gene polymorphism on superovulation traits and suggested that the mutation could provide a basis for cattle breeding and improving animal fertility.