Prevalence of Extended Spectrum Beta-Lactamase Phenotype and blaCTX-M-I, blaSHV, and blaTEM Genes among Uro-Pathogenic Escherichia coli Isolates, in Egypt

摘要

Copyright © 2022 by Razi Vaccine Serum Research InstituteOne of the main reasons for recurrent urinary tract infections (UTIs) could be explained as follows: it is well approved that uro-pathogenic E. coli (UPEC) isolates have the ability to persist in urothelial cells, therefore, it can cause recurrent UTIs. The prevalence of extended-spectrum beta-lactamase (ESBL) has been considered a global health issue. It is well documented that the blaCTX-M, blaSHV, and blaTEM are the prevailing betalactamase. Therefore, the current study was designed to determine the ESBL production and prevalence of blaCTX-M1, blaSHV and blaTEM genes among UPEC isolated from 3 hospitals during 2020-2021, Egypt. Total of 111 isolates were obtained from three different hospitals in Egypt during the years 2020-2021. The antibiotic susceptibility testing was conducted according to CLSI advice. The combine disk was used for phenotypic ESBL production. The MIC of ceftazidime was conducted with the micro-broth dilution test. The cloxacilin containing MH agar was used to detect the AmpC-lactamase. The PCR assay was used to detect the blaCTX-M-I, blaSHV and blaTEM genes. The results revealed that in the broth microdilution method, 103 (92.7) isolates showed MIC≥1, and in the combined disk method, 89 (80.1 of all) were ESBL producers. On the other hand, among 91 ceftazidime resistant isolates, 86 (77.4 of all) were ESBL positive. The difference between the 2 methods for ESBL confirmation was not significant. The results of MIC confirmed the disk diffusion for determination of phenotypic test for ESBL production. The prevalence of blaCTX-M-I, blaSHV and blaTEM genes among ESBL producers was 77.4 (n=86), 47.4 (n=53) and 2.4 (n=2) respectively. These enzymes were amplified in a wide range of MIC to ceftazidime. The prevalence of MDR UPEC and ESBL positive isolates was high in military hospitals in Egypt. The majority of UPEC isolates amplified blaCTXM-I and blaSHV type β–lactamases. One-third of isolates were positive for both these genes. There was no relation between MIC range of ceftazidime and presence of beta-lactamase genes.