Next-generation sequencing of prostate tumors provides independent evidence of xenotropic murine leukemia virus-related gammaretrovirus contamination

摘要

In 2006, xenotropic murine leukemia virus (MLV)-related gammaretrovirus (XMRV) was isolated from prostate cancer tissue (9). However, subsequent studies have yielded conflicting and controversial results, with widespread detection of XMRV suggested to be the result of contamination with mouse DNA (3). While recent work has focused on PCR-based targeted detection of XMRV (10), the advent of next-generation sequencing (NGS) means that it is now possible to interrogate the entire genomes and transcriptomes of human samples for the unique genomic signatures of thousands of viruses (1, 5, 6).