Brucella abortus biovar 3 is an uncommon cause of human brucellosis in Spain, where Brucella melitensis has accounted for 97.5 of all cases. B. abortus biovar 3 was detected in specimens from four patients in Caceres (west-central Spain) with a febrile syndrome associated with arthralgia and myalgia and from one patient in Lugo (northwestern Spain) with orchiepididymitis (March 2004 and April 2005). For tracing, these isolates were studied by biotyping, analysis of 16S rRNA genes, rpoB, gyrA, parC, and housekeeping genes, multilocus variable-number tandem-repeat analysis (MLVA-16), and analysis of hypervariable octameric oligonucleotide fingerprints (HOOF-prints).
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