Routine identification of clinical isolates of anaerobic bacteria: Matrix-assisted laser desorption ionization-time of flight mass spectrometry performs better than conventional identification methods

摘要

Recently, Justesen et al. (1) compared identification results of the Bruker matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) system and the Shimadzu/SARAMIS system on 290 consecutively collected clinical anaerobic strains. The Bruker system identified 67 of the isolates correctly to the species level, and the Shimadzu system correctly identified 49. These results are somewhat contradictory to our findings of 2010 (5), with 51 and 61 correct species identifications for Bruker and Shimadzu, respectively. This difference may be explained by the higher percentage of Gram-positive anaerobic cocci (30) and lower number of Bacteroides species in our study (22) combined with the better performance of the Bruker system on Bacteroides species and the better performance of the Shimadzu system on anaerobic Gram-positive cocci (4, 5).