Misdiagnosis of Mycobacterium brumae Infection.

摘要

We read with interest the paper "Catheter-Related Bloodstream Infection Caused by Mycobacterium brumae" by Lee and colleagues (4) because we had described this species in 1993 and no other isolates had been reported from this first description (5). So we contacted the corresponding author, Dr. Han, who gently sent us the M. brumae strain causing the bloodstream infection (MDA0695 strain). In order to confirm the identification of the MDA0695 strain, we performed a total of 24 biochemical tests and growing characteristics by following standard procedures (8). In vitro antibiotic susceptibility was performed in Middlebrook 7H10 agar by the proportion method (8). The M. brumae type strain and the MDA0695 strain were in disagreement in the following eight phenotypic characteristics: colony pigmentation (Fig. 1), growth at 42degC, growth in the presence of 5 NaC1, nitrate reductase (2 h), arylsulfatase (3 days), iron uptake, use of inositol as a sole carbon source, and sensitivity to streptomycin (4 mug/ml) (Table 1).