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外文期刊>Journal of Clinical Microbiology
>Effect of sample storage conditions on culture-independent bacterial community measures in cystic fibrosis sputum specimens.
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Effect of sample storage conditions on culture-independent bacterial community measures in cystic fibrosis sputum specimens.
Culture-independent approaches to studying the microbiota of the airways of cystic fibrosis (CF) patients have employed analysis of the bacterial 16S rRNA gene through Sanger sequencing of clone libraries (5, 6, 12), terminal restriction fragment length polymorphism analysis (8,11), microarray hybridization (3, 7), or pyrosequencing (2, 5) and have revealed the presence of far more complex bacterial communities than has been appreciated with culture-based methods. Expectorated sputum has been used in many of these studies and, when collected properly, is estimated to contain a relatively low abundance of bacteria from the oropharynx (9). However, sputum samples typically require transportation and/or storage before culture-independent analysis.
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