Three hundred sixty Enterobacteriaceae and nonfermenting gram-negative bacilli, isolated during one week in May 2004 at five hospitals in Amsterdam, The Netherlands, were evaluated for the presence of extended-spectrum beta-lactamases (ESBLs). A prevalence of 7.8 was found, in contrast to the 1 observed in 1997. CTX-M ESBLs dominated, and four types were identified in 18 isolates.
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