Real-time TaqMan PCR for yersinia enterocolitica detection based on the ail and foxA genes

摘要

Yersinia enterocolitica, a cause of emerging enteric infections, is a foodborne pathogen associated with various enteric and systemic syndromes, e.g., diarrhea, enteritis, and enterocolitis. Therefore, the detection of this pathogen has important significance. Previous real-time PCR for detection of Y. enterocolitica was primarily based on the ail gene; biotype 1A nonpathogenic strains were not included (1-3). However, recent studies (4, 5) showed that biotype lAystS-positive strains are potentially pathogenic and related outbreaks were reported. We therefore designed a TaqMan real-time PCR method for detection of both pathogenic and nonpathogenic Y. enterocolitica strains.