Rapid identification and validation of specific molecular targets for detection of Escherichia coli O104:H4 outbreak strain by use of high-throughput sequencing data from nine genomes.

摘要

In May 2011, an enteroaggregative verocytotoxin-produc-ing strain of Escherichia coli O104:H4 caused an outbreak of hemolytic uremic syndrome in Germany. Although traditional culture and phenotypic tests can identify the outbreak strain, rapid molecular testing is useful for timely diagnosis. While multitarget PCR assays have been reported by Bie-laszewska et al. (1) and Cui et al.