Molecular detection method for all known genotypes of TT virus (TTV) and TTV-like viruses in thalassemia patients and healthy individuals.

摘要

High sequence diversity among TT virus (TTV) and TTV-like viruses (TTLVs) is an obstacle to PCR detection of the entire spectrum of existing genotypes. To determine which primers are needed to detect all know genotypes of these viruses, an alignment of commonly used primer sequences with sequences of all known viral genotypes was performed to predict theoretically amplifiable genotypes. The results predicted that all TTV genotypes (except genotype 21), all SEN virus (SENV) genotypes (A to H), and three known TTVL minivirus (TLMV) groups would be detectable by TTV primers NG054/ NG147/133/132 (NG) and TT6/7/8/9 (TT), combined with two TLMV primer sets (TLMV-S and TLMV-L).