Chlamydia pneumoniae in peripheral blood mononuclear cells isolated from individuals younger than 20 years or older than 60 years.

摘要

Serological studies of Chlamydia pneumoniae in humans have shown a correlation between the age of a population and the number of individuals positive for C. pneumoniae antibodies. Grayston (1) has reported that individuals younger than 30 had 50 seropositivity and that individuals older than 60 had 80 seropositivity. We searched for C. pneumoniae DNA in peripheral blood mononuclear cells (PBMCs) isolated from two groups: those younger than 20 years of age and those older than 60 years of age.PBMCs were prepared using either Sigma Histopaque-1077 (Sigma Chemical Company, St. Louis, MO) or traditional Fi-coll-based methods. Isolated PBMCs were resuspended in 4 ml of RPMI medium purchased from Invitrogen (Carlsbad, CA). Aliquots (1 ml) were held at-80degC for later analysis.