Strong and Selective Binding of Amiloride to an Abasic Site in RNA Duplexes: Thermodynamic Characterization and MicroRNA Detection

摘要

Small ligands that can bind to nucleic acids have been of great interest because of their promising abilities as therapeutical candidates to modulate or inhibit the crucial functions of nucleic acids. These ligands have also been used as powerful tools for the detection of nucleic acids in solutions as well as in various platforms such as gels and microarrays. The non-covalent binding modes of ligands with DNAs are broadly divided into two categories, that is, intercalation and minor groove binding.