Fabrication of ssDNA/Oiigo(etliyIene glycol) Monolayers and Complex Nanostructures by an Irradiation-Promoted Exchange Reaction

摘要

The macromolecular structure of DNA, its ability to hybridize, the diversity of non-natural nucleotides with unique functional groups that are available to perform chemistry on it, and enzymes that are capable of manipulating its sequence, structure, and topology provide rich opportunities for its use in clinical diagnostics, biosensors, gene therapy, and drug delivery. Some of these applications rely on the immobilization of single-stranded DNA (ssDNA) onto a solid support, which is subsequently used for binding and detection of its complementary ssDNA target or for the recognition of DNA binding proteins. A commonly used method for immobilization of ssDNAs is to functionalize them with a terminal reactive group that is selective for the surface of interest.Depending on the particular application, immobilization can be performed either homogeneously over the entire surface or lithographically, resulting in an array of ssDNA spots.