Electrochemical Gene-Function Analysis for Single Cells with Addressable Microelectrode/Microwell Arrays

摘要

Cell arrays enable the simultaneous detection of single or grouped cells for the analysis of individual cellular functions under the same experimental conditions, which can greatly reduce experimental error, accelerate detection rates, and help elucidate cell heterogeneity. Most cell array systems are based on fluorescence detection methods, which usually have high sensitivity and for which many measurement tools are commercially available. An alternative elec-troanalytical detection method offers substantial advantages, including high compatibility with micro- and nanomachining technologies, system simplicity, small size, reliability, ease of use, and high sensitivity. Among the various systems that are based on electrochemical detection, individually addressable electrode arrays have been used recently for simultaneous multianalyte detection. But all these methods were realized using individual addressing by connecting each electrode to a corresponding bond pad in the 1:1 mode for performing the electrochemical measurements. However, the integration of a large number of detection electrodes is difficult, since the space for bond pads on the chip border is limited.