Changing the Regioselectivity of the Tryptophan 7-Halogenase PrnA by Site-Directed Mutagenesis

摘要

For many years, haloperoxidases were the only type of halogenating enzymes known. Haloperoxidases (heme-and vanadium-containing) catalyze the formation of hypoh-alous acids, which diffuse out of the active site and then react with substrate. Perhydrolases catalyze the formation of peracids, which react outside of the active site with halide ions to form hypohalous acids.