High-Throughput In Vitro Glycoside Hydrolase (HIGH) Screening for Enzyme Discovery

摘要

There has recently been a renewed interest in converting the carbohydrate content (cellulose and hemicellulose) of ligno-cellulosic biomass to biofuels and biochemicals. This conversion requires depolymerization of the carbohydrate content of biomass to fermentable monosaccharides by using glycoside hydrolases (GHs). Despite the vast number of GHs deposited in publicly available databases, 90 of these enzymes are not characterized and many are unlikely to be suitable for industrial applications because of low activity and stability under processing conditions.