Optimal Culture Conditions and Isolation of a l-Carrageenase-producing Marine Bacterium

摘要

A bacterial strain capable of hydrolyzing carrageenan was isolated from the coast of Busan in Korea. The isolated strain (HS5322) is aerobic, gram-negative, rod-shaped, and motile. Comparison of the 16S rDNA of the isolate with that of known Pseudomonas sp. showed that sequence similarity was at most 95, implying that the isolate is a new Pseudomonas species. The organism grew optimally at NaCl concentrations of 2.0 to 2.5. The optimum temperature and pH for carrageenase production in a 72-h flaskculture containing 1 carrageenan was 20°C and pH 8.5, respectively. Of the synthetic substrates tested, the highest enzyme activity was obtained with p-nitrophenyl p-D-galactopyranoside.