Development of ten microsatellite markers for Alibertia edulis (Rubiaceae), a Brazilian savanna tree species

摘要

Ten microsatellite markers were developed using next-generation sequencing data for Alibertia edulis (Rubiaceae), a widely distributed species typical of Cerrado (Brazilian savanna) vegetation. The markers were polymorphic in the two populations analyzed. The numbers of alleles, and observed (H-O) and expected (H-E) heterozygosities per polymorphic locus ranged from 2 to 11, 0.091 to 1.0, and 0.100 to 0.937 respectively. The SSR loci demonstrated moderate to high polymorphism values in both populations analyzed, with PIC values ranging from 0.26 to 0.91, and total allele numbers ranging from three to 16. The inbreeding coefficient values were generally higher in the Piaui population (ranging from -0.593 to 0.762) than in the Mato Grosso population (ranging from -1 to 0.575). The differences observed between those disjunct populations suggest they harbor different alleles, which has implications for Cerrado conservation strategies. Those loci will be useful for population studies of A. edulis.