Abscisic acid induced somatic embryogenesis in immature embryo explants of coconut (Cocos nucifera L.).

摘要

Nodular callus was obtained from 7- to 9-month-old immature zygotic embryos of coconut on a medium containing 24μM 2,4-D. As a novel approach, abscisic acid (ABA) at 2.5-7.5μM was incorporated into the culture medium for 3-7 weeks to induce somatic embryogenesis. Alternately, callus was subcultured at 5-weekly intervals on media containing gradually reducing concentrations of 2,4-D to induce somatic embryogenesis. Incorporation of ABA enhanced the production of somatic embryos. Application of 2.5-5μM ABA for 5 weeks was found to be effective. A large number of somatic embryos developed on media containing ABA formed normal shoots and complete plants, as compared to those produced in the media with low levels of 2,4-D.