Fermentative Production of Injectable‐Grade Calcium Gluconate

摘要

AbstractOptimized economical procedures for the submerged batchwise (250‐L scale) production and recovery of injectable‐grade calcium gluconate are devised. While the optimal parameters of production envisaged the use ofAspergillus niger, salts‐fortified dextrose‐rich production medium, pH 6.5±0.1, 29±1°C, 250±10 rpm, 1.0‐1.5 vol of air (vol of medium)−1min−1aeration rate over 24±2h duration, and intermittent neutralization with calcium carbonate, the optimal recovery procedure consisted of charcoal decolorization, vacuum filtration, methanol‐aided precipitation, centrifugation, vacuum drying, and pulverization to provide 85 recovery of calcium gluconate, passing Indian/British pharmacopeial tests for the injectab