Effect of Artificially Inserted Intron on Gene Expression inSaccharomyces cerevisiae

摘要

ABSTRACTThe intron of the yeastRP51Agene was cloned with precision using the polymerase chain reaction (PCR) amplification method, and then inserted into several different positions of the yeastURA3gene as well as thePGK–lacZfusion gene without introduction of additional exon sequences. Analysis of transcripts of these genes showed that an intron inserted near the transcription start site of the gene was spliced out efficiently, whereas the same intron sequences inserted 200 bp or further downstream of the start site were not, resulting in a reduced level of mRNA. These results explain why intron-containing genes in yeast usually have an intron near the 5′